International Journal of Herbal Medicine
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E-ISSN: 2321-2187, P-ISSN: 2394-0514   |   Impact Factor: RJIF 5.20 NAAS Rating: 3.20
International Journal of Herbal Medicine
Vol. 1, Issue 2, Part A (2013)

Gossypol Analysis in Bt and Non-Bt Cotton Seed Extracts by High-Performance Liquid Chromatography (HPLC)

Author(s): R.Chandrashekar, Rao Kudle, P.Jyothi Chaitanya, N.Lakshmi Bhavani
Abstract: Present investigation on gossypol content of various seed extracts of Bt and non-Bt of cotton varieties was conducted through an optimized High-Performance Liquid Chromatography (HPLC) on a C18 column (4.6 mm × 250 mm, 5 mm particle) with methanol–0.5% acetic acid aqueous solution, 90: 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. The results revealed slightly differences among the Bt and non-Bt varieties of cotton in gossypol content including the Gossypium hirusutum (non-Bt) and Bt varieties of cotton.
Pages: 53-58  |  1190 Views  9 Downloads
How to cite this article:
R.Chandrashekar, Rao Kudle, P.Jyothi Chaitanya, N.Lakshmi Bhavani. Gossypol Analysis in Bt and Non-Bt Cotton Seed Extracts by High-Performance Liquid Chromatography (HPLC). International Journal of Herbal Medicine. 2013; 1(2): 53-58.
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International Journal of Herbal Medicine