Vol. 4, Issue 2, Part A (2016)
Neem seed extract may be used as topical ointment to treat wound infections caused by Pseudomonas aeruginosa
Author(s): Chatterjee Arijit
Abstract: Pseudomonas aeruginosa is an important nosocomial pathogen, usually resistant to many antibiotics. This study was aimed to determine bactericidal effect of the extract of ‘Neem Seeds’ (Azadirachta indica) on Pseudomonas aeruginosa isolated from wound infection patients. In this study, 60 strains of Pseudomonas aeruginosa isolated from patients suffering from wound infections. Undiluted extract of neem seeds obtained after grinding & 106 CFU/ml was performed and the surviving bacteria were detected by culture on PIA (Pseudomonas Isolation Agar). Minimum Inhibitory Concentration was determined by agar dilution method (10% to 50% concentration of neem seeds extract in 20 ml of molten nutrient agar by inoculation of 104 CFU /spot on MHA. Out of 60 strains of Pseudomonas aeruginosa, 36 (60%) were killed after 1hr exposure to neem seeds extract, 12(20%) were killed after 2 hrs exposure, 8(13.34%) were killed after 4 hrs & remaining 4(6.66%) were killed after 8 hrs exposure respectively. All Pseudomonas strains were killed by extract of the neem seeds. On agar dilution, 51 (85%) of strains were inhibited at 10% concentration of neem seeds extract, 7 (11.67%) at 20% and remaining 2(3.33%) were inhibited at 30% concentration of the extract. The results of the above study clearly indicated that direct extract obtained from grinding the neem seeds, has bactericidal activity on Pseudomonas aeruginosa.
How to cite this article:
Chatterjee Arijit. Neem seed extract may be used as topical ointment to treat wound infections caused by Pseudomonas aeruginosa. International Journal of Herbal Medicine. 2016; 4(2): 25-26.