Author(s): Agus Santosa, Bakhtiar Noor Abidin and Kurniawan

Abstract: Nigella sativa is commonly used as a traditional medicine since ancient times in which some of its substances are believed to contain anti-oxidants and anti-inflammatory. This research aimed to determine the anti-bacterial activities of ethanol Nigella sativa extract from subjects with diabetic foot ulcers in vitro. It was an experimental research; Nigella sativa extraction was done with n-hexane, ethyl acetate, and ethanol, then antibacterial tests were conducted on the extraction result. Bacterial samples were taken from subjects with diabetic foot ulcers and were cultured in PCA media. Anti-bacterial activities were seen from the diameter of the bacterial inhibitory zone. Data were analyzed using one-way Anova. Results from the culture of diabetic foot ulcers specimens showed apoly microbial growth. The prevalence of Gram-negative bacteria was found to be more than the prevalence of Gram-positive bacteria. The test results revealed that ethanol extracts of Nigella sativa were able to inhibit bacterial growth. Ethanol extract of Nigella sativa had the greatest effect on bacterial growth with the average inhibitory zone of 257.5 mm2 (p<0.05). We conclude that the ethanol extracts of Nigella sativa have powerful anti-bacterial activities.