Author(s): Martin Kalumbi

Abstract: Mangifera indica leaves play a major role in combating antibiotic resistance. They are processed using various methods to yield powder for phytochemical extraction. Present study was conducted to determine effect of processing method and concentration of leaf extract on their antibacterial activity against S. aureus. Four by four factorial design was employed involving two factors namely processing method (mortar and pestle, blender, laboratory mill and stone) and concentration of extract (6.25, 12.5, 25 and 50 mg/ml). Antibacterial activity was determined by disc method. Both factors influenced antibacterial activity, but there was no interaction effect (p = 0.353) of the factors. Highest inhibition zones were recorded for Laboratory mill (14.5mm) and 50 mg/ml concentration (16.7mm). Inhibition zones for other methods were similar (p = 0.624). MIC was 6.25mg/ml while MBC was 12.5mg/ml. The study has provided evidence on effectiveness of laboratory mill compared with other processing methods in Malawi.