Vol. 7, Issue 2, Part A (2019)
Pharmacognostic evaluation, fluorescence and TLC analysis of Peltophorum pterocarpum (Dc.) backer ex heyne leaves
Author(s): Dharmasoth Rama Devi, Venugopalareddy Mekala and B Ganga Rao
Abstract: Present study is to evaluate the Pharmacognostic, Florescent Analysis and Thin Layer Chromatography of Peltophorum pterocarpum leaf. Fresh and shade dried leaves were selected and powdered for this study. Physicochemical parameters were performed as per WHO guidelines. The powder was investigated by morphology and Florescent Analysis (FA), Thin Layer Chromatography (TLC) was analysed according to method described by Chase and Pratt (1949) and Kokoshi et al., (1958). The results of physico-chemical parameters such as determination of total ash, water soluble ash, insoluble ash, loss on drying, alcohol soluble extractive values are tabulated. This crude extract (leaf powder) showed the characteristic physicochemical values like 10.27% (total ash), 6.53% (water soluble ash), 12.5 % (acid insoluble ash), and 0.15% (moisture content). Methanolic extract of Peltophorum Pterocarpum was used for the fluorescent and TLC analysis. Fluorescent characters was observed varied colours like green, Yellowish green, dark green, Reddish brown and Yellowish brown, Blackish brown, Bluish brown, Dark brown, Light brown under different chemical treatments on methanolic extract used for the study. TLC showed nine different compounds with different Rf values, confirmed the presence of different compounds in the plant. From the results the Peltophorum Pterocarpum leaf have potential bioactive constituencies.
How to cite this article:
Dharmasoth Rama Devi, Venugopalareddy Mekala and B Ganga Rao. Pharmacognostic evaluation, fluorescence and TLC analysis of Peltophorum pterocarpum (Dc.) backer ex heyne leaves. International Journal of Herbal Medicine. 2019; 7(2): 13-18.