Author(s): Neha Pandey and Janmejay Pant

Abstract: Medicinal plants are best remedies used as alternative tools for the prevention and treatment of many ailments. Rosaceae (the rose family) is a medium-sized family of flowering plants, including about 2830 species in 95 genera. Rosaceae includes herbs, shrubs and trees. Most species are deciduous, but some are evergreen. In this study, the physicochemical evaluation; ash values, namely total ash, water-soluble ash, and acid-insoluble ash, and extractive values; namely ethanol-soluble extractive and water-soluble extractive values, moisture content by using loss of drying method, total phenolic and total flavonoid contents in Pyrus pashialeaves and flowers were used for illustrate the quality as well as purity and also investigated the pharmacological effect; namely antioxidant effect by using DPPH free radical assay. The total ash, water-soluble ash, and acid‑insoluble ash in Pyrus pashia leaves was found to be 5%, 3.1%, and 1.9%, respectively. In other side in Pyrus pashia flowers the total ash, water-soluble ash, and acid‑insoluble ash in Pyrus pashia leaves was found to be 5.12%, 3.05%, and 2.07%, respectively. The water-soluble extractive value was higher than alcohol-soluble extractive value in this study, and it was found to be 4.53% in leaves and 4.65% in flowers. The moisture content of the crude drug of leaves and flowers was found below 6.33% and 8.20%, respectively. The total phenolic content in the leaves and flowers extracts was found 180 ± 7 mg and 70 ± 9 mg of Gallic acid equivalent weight/g of extract, respectively, and the concentration of flavonoids in leaves and flowers extract of Pyrus pashia was found 371 ± 12 and 130± 10 mg of Quercetin equivalent weight/g of extract, respectively. The percentage inhibition of scavenging activities of the Pyrus pashia leaves and flowers showed 51.60% and 43.69% DPPH inhibition at 50 µg/mL concentrations. Whereas ascorbic acid showed 66.25% DDPH inhibition at 50 µg/mL.