Vol. 2, Issue 6, Part A (2015)
The use of alfalfa root instead of streptolysin O in ASO diagnostic test
Author(s): Hamid Chegni, Mojgan Oshaghi, Behnaz Gharegozlou, Mehrangiz Kaviany
Abstract: Alfalfa (Medicago sativa) has been used as nutritious animal fodder for over years. Streptolysin O (SLO) is one of the several toxic immunogenic exoenzyme produced by group A β-hemolytic Streptococci. ASO is a routine test for post streptococcal infections diagnosis. This study explored the application of Alfalfa root extract instead of SLO. Hemolytic effect of the Methanolic extract of Alfalfa roots was determined by using RBC suspension. Immunological properties of Alfalfa root extract was evaluated by some serological techniques. This study showed that hemolytic effect of Alfalfa roots extract is similar to SLO and the ASO was inhibited hemolysis activity of the extract. Ouchterlony test demonstrate its antigenic similarity and Slide Agglutination Inhibition and neutralizing methods prove its efficiency similarity with SLO. There is significant similarity of antigenic property in both Alfalfa extract and SLO. Thus purified extract of Alfalfa can used as alternative suitable reagent instead of SLO for ASO diagnostic test.
How to cite this article:
Hamid Chegni, Mojgan Oshaghi, Behnaz Gharegozlou, Mehrangiz Kaviany. The use of alfalfa root instead of streptolysin O in ASO diagnostic test. Int J Herb Med 2015;2(6):10-12.