Vol. 5, Issue 5, Part C (2017)
Effect of Hemidesmus indicus R.Br. root extract on urinary tract infection causing bacteria
Author(s): Sarita Das, Priyanka Naik and Preetilata Panda
Abstract: Hemidesmus indicus is a well known medicinal plant used in Ayurveda for treating many diseases. But there are fewer reports on the uroprotective activity of H. indicus root extract. So, the present study was carried out to investigate the effect of the methanolic extract of H. indicus root (MHI) against uropathogenic bacteria i.e. Escherichia coli and Klebsiella pneumoniae. Multidrug resistant bacterial strains, clinically isolated from patients by the Department of Microbiology, MKCG Medical College, Berhampur, were used in the study. Antibacterial activity of MHI was tested against the above-mentioned bacteria by using four methods, viz; disc diffusion, agar well diffusion, modified agar well diffusion and cfu/ml determination in control and MHI treated bacteria by using spread plate and absorbance methods. In disc diffusion method, MHI was more effective against K. pneumoniae in comparison to E. coli whereas, in agar well and modified agar well diffusion method, MHI was comparatively more effective against E. coli than K. pneumoniae. The cfu/ml in MHI treated bacteria was reduced in a dose dependent manner in comparison to wild bacteria as evident from the results of spread plate and absorbance method. So, MHI can be used against multidrug resistant strains causing UTI either individually or as combination therapy along with the specific antibiotics.
How to cite this article:
Sarita Das, Priyanka Naik, Preetilata Panda. Effect of Hemidesmus indicus R.Br. root extract on urinary tract infection causing bacteria. Int J Herb Med 2017;5(5):160-168.