Author(s): Naynika Patel and JSS Mohan

Abstract: Since many centuries Chinese and Indian folk medicine been using Piper betle leaves as medicine and different research works and traditional medicinal uses which signify the tremendous potential of “Piper betle” to come out as Green medicine. In the present study, Piper betel varieties Banarasi and Bengali, Calcati, Kapoori and Malbari was extracted with sequential extraction method using n-Hexane, Ethyl acetate, Methanol and Distilled water. The crude ethyl acetate extract of Banarasi and Bengali was separated using analytical thin-layer chromatography (TLC).The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against bacteria using TLC bioautography approach. Antimicrobial activity was visualized on TLC after spraying with MTT and triphenyltetrazolium chloride reagents which screen antioxidant and antimicrobial compounds, respectively.Extraction of the bioactive constituents from the leaf extract of Piper betle varieties Banarasi and Bengali was carried out and characterized by separation techniques like HPTLC and structure elucidation spectroscopic analysis techniques like 1H-NMR and FT-IR. Phytochemical screening of different extractions showed presence of phenols, flavanoids and the isolation purification active principles as Hydroxy Chavicol, Chavicol, Eugenol from leaves extract when subjected to physical, chemical and spectral identification.