Author(s): Gökçe Şeker Karatoprak, Selen İlgün and Müberra Koşar

Abstract: Alchemilla mollis (Buser) Rothm. (Rosaceae) that grows in Turkey was examined for its different biological activities. The chemical composition of 50% methanol, water and deodorized water extract were determined spectrophotometrically (total phenol, total flavonoid and total flavonols). The antiradical activity was determined by DPPH^• (1,1-diphenyl- 2-picrylhydrazyl) and ABTS^+• (2,2’-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity assays. Antimicrobial activity was investigated by Agar Dilution Method. Finally the cytotoxic effect was evaluated on MCF 7 (human breast adenocarcinoma cell line) cell line with using Sulforhodamine B (SRB) method. The deodorized water extract showed higher antiradical activity in DPPH^• method whereas water extract in ABTS^{+ •} method. Water extract and deodorized water extract inhibited the viability of the MCF 7 cells by 70% at a concentration of 125.0 μg/mL. These results show that A. mollis may be a source of antiradical, antimicrobial and cytotoxic agent to support the medical use.