Author(s): Prasetyorini Djarot

Abstract: The Annona muricata or soursop fruit has long been used in folk medicine for the treatment of variable ailments including hyperuricemia. Recent study has successfully formulated the soursop fruit juice into more practical preparation of instant granule drink. The aim of this study was toinvestigate the antihyperuricemic activity of instant granuleformulated from soursopfruit juice on hyperuricemia induce Sprague Dawleys rat. The soursop granulewas produced from fresh fruit juice with the addition of carboxymethyl cellulose (CMC), citric acid, sucralose and maltodextrin. Different doses of granule (90mg/200g b.wt.180 mg/200g b. wt. and 270 mg//200g b. wt.) were administeredorally to potassium oxonate-induced Sprague Dawley rats. The uric acid level of rats wasmeasured subsequently on the day 4, 8, and 12 of treatment period. The soursop fruit juice produced bright white granule with tipically fresh sour sweet taste, 2.9% water content (<5%), 1.91% ashcontent, 3.13 ml/s solubility, 29.3% angle of repose, and stable in the storage at 15⁰C for two months. The soursop granule have shown significant antihyperuricemic activityin both male and female Sprague Dawley rats. The highest percentage of uric acid level decrease occurs at day 8in male rats treated with 270 mg soursop granule and at day 12 in female rats treated with 180 mglevel of soursop granule respectively. The overall the results show thatthe soursop granule has a potent as antihyperuricemic natural medicine which works in gender and time dependent manner, and can be developed to substitute synthetic antihypertensive drugs.