Author(s): Ranjith D, Senthil Murugan S, Nisha AR, Muhsina Rahman M, Sivan VV and Juliet S

Abstract: Standardization of herbal formulation is essential in order to assess the quality of drugs for therapeutic value based on the concentration of their active principles. In the present study herbal formulation containing rhizomes of Curcuma longa and leaves of Diploclisia glaucescens were standardized according to the set guidelines from world health organization. The parameter considered were organoleptic, physicochemical, pH range, fluorescence analysis, swelling and foaming index, preliminary phytochemical analysis, heavy metal testing and acute oral toxicity studies of the formulations. The results indicated, all the pharmacognostic parameters were as per the limit set by world health organization and the formulation showed maximum phytocon students. Upon oral toxicity, the extract shown to be safer at 2000 mg/kg body weight both in terms of haematological, biochemical parameters. In conclusion, these data of herbal formulation can be used as reference monographs for further detailed study of pharmacological activity.