Author(s): Anant Babu Marahatta, Aashish Aryal and Ram Chandra Basnyat

Abstract: Nepal, being a land of diverse topography with different weather condition, the types of secondary metabolites/phytoconstituents of the locally grown Annona squamosa plant (SARIFA, सरीफा in Nepalese language) could be diverse. That is why, study of the phytochemical and nutrirional analysis and biological activity of such plant grown in the local weather is extremely essential. As this plant is very popular due to its wide application as traditional medicine from ancient times to this day, we have chosen its type grown in the subtropical region of Nepal as experimental specimen and extracted the contents of its leaves and bark in methanol and hexane solvents separately by Soxhlet extraction method. The phytochemical screening process has unveiled alkaloids, polyphenols, flavonoids, and tannins in either of these extracts. The total phenolic and flavonoid content for the methanol extract of leaves and bark are estimated as 217.82 and 160.48 mg/g GAE and 66.92 and 76.50 mg/g QE respectively. In DPPH free radical scavenging test, the methanol and hexane extract of leaves shows the highest and second highest antioxidant activities. In α-amylase inhibition assay, the methanol extract of bark shows considerable amount of antidiabetic activities. The significant ZOI, MIC and MBC values for the methanol extracts of leaves and bark confirm their abilities to cure the diseases caused by Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus bacteria. The major chemical compounds: Palmitic acid and Geranyl linalool in leaves and Isopimarol and Sandaracopimarinol in bark are also predicted by GC/MS analysis.