Author(s): K Saranya, Anupa Mary Aji, S Keerthana, NG Ramesh Babu, C Sivaraj and P Arumugam

Abstract: Hypericum hookerianum commonly known as Hooker’s St. John’s Wort belongs to the family Hypericaceae. It helps in treatment of chronic diseases, especially schizophrenia. The study has been done on the methanol leaf extract of the plant. It aimed at evaluating the antioxidant and antibacterial activities of hypericin fraction. The maximum 1, 1- diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity of methanol extract of leaves of hypericin fraction was 59.36 ± 0.49% at 120 µg/mL concentration and the IC₅₀ was 79.05 µg/mL concentration. The maximum superoxide radical scavenging activity of hypericin extract was 36.05 ± 0.09% at 120 µg/mL concentration and the 50% inhibitory concentration (IC₅₀) was 166.41 µg/mL concentration. The maximum Fe³⁺ reduction was 84.81 ± 0.12% with the 50% reduction concentration (RC₅₀) of 20.05 µg/mL and the maximum Mo6+ reduction was 99.25 ± 0.35% at 120 µg/mL concentration with the RC₅₀ of 10.14 µg/mL concentration.