Author(s): Sandip S Kale, Suresh Kale and Padma Dandge

Abstract: The present study was designed for isolation, purification, and chemical characterization of components from Cassia fistula responsible for antibacterial activity. The crude extract of leaves of Cassia fistula prepared using methanol as a solvent and phytochemical evaluation was done. An invitro antibacterial assay was conducted to evaluate the inhibitory activity of methanol extract against E. coli and B. cereus. The methanol extract was subjected to column chromatography with different mobile phases followed by TLC. HPLC of fractions showing maximum inhibitory activity against E. coli and B. cereus was performed. The chemical characterization of isolated compounds was achieved using FTIR and ¹H NMR spectroscopy. Screening of antibacterial activity of methanol extract showed marked activity against both gram-positive and gram-negative bacteria. FTIR and NMR analysis data show the presence of various functional groups that correspond to potential bioactive compounds from phenolics (Rhein, Catechin), triterpenes (Hopane), and Flavonoids (Amentoflavone). This plant formulation may thereby provide an alternative solution to the current problem associated with the use of antibiotics.