Despite all of the control approaches, tuberculosis (TB) is a major cause of death worldwide and one-third of the world’s population is infected with TB. Plant-derived medicines have been used in traditional medicinal system for the treatment of many ailments worldwide. From last number of years, plants have advantageous in different type of diseases producing in human beings. The present aim to carry out the evaluation of the antimycobacterial activity of selected eleven medicinal plants. Three different extracts were prepared and evaluated for its antimycobacterial activity against Mycobacterium smegmatis using Mycobacterial Growth Indicator Tube (MGIT) assay. The MGIT assay consists of liquid broth medium that is known to yield better recovery and faster growth of mycobacteria. Isoniazid was used as standard antituberculosis drug. The percentage for anti-mycobacterial smegmatis activity among tested eleven medicinal plants, aqueous extract of Oscimum sanctum, Adhatoda vasica, Leptadenia reticulata and Cocculus hirsutus shows good antimycobacterial activity. Among these four Leptadenia reticulata and Cocculus hirsutus shows potent inhibition as compared to isoniazid. Thus, its result supports the uses of these plants in traditional medicine and also helps to cure and prevent tuberculosis. It can further have studied using more specific methods for antimycobacterial activity.