Nanotechnology belongs to one of the areas of modern science, demonstrated numerous positive developments in the realm of medical research, basically deals with the particle size ranging between 1-100 nm. Out of so many methods for developing the metal nanoparticles, the green approach for metal nanoparticles preparation is less hazardous, eco-friendly and cost effective. According to various literature studies, plants belonging to Citrus species have enormous potential as anticancer and antioxidant rich food items. Therefore our present research work aims at the Citrus species (lemon, pummelo and the aroma king lemon) mediated Metal nano-particle synthesis followed by their characterization and determining its antibacterial property of against Staphylococcus aureus bacterium. According to the results of physical characterizations, all the three Citrus mediated nanoparticles show UV-Vis spectra within 520-530 nm. And have the particle size generated within 1-10 nm. Several functional groups are present in the produced nanoparticles, according to an FTIR analysis. The in-vitro antioxidant efficacy was maximum shown by the aroma king lemon. This overall results supports the innate antioxidant properties of Citrus species plants that may further supports its reducing property for nanoparticle synthesis.