Acne is an inflammatory skin disease that occurs due to blockages in polysebase and inflammation that are caused by bacteria. Propionibacterium acnes are common pus-forming microbes responsible for the development of acne. Medicinal plants play an important role in the development of potent therapeutic agents. Plant based drugs provide outstanding contribution to modern therapeutics as a source of many valuable secondary metabolites which serves as plant defense mechanisms against predator such as microorganism, insects and herbivores which have been proved to be potentially active compounds. There is a tremendous increase in search of antimicrobial plant extracts due to the fact that the resistance offered against antibiotic by the microorganism, in short the effective life span of any antibiotic is limited. The herbal gel preparation was formulated based upon traditional knowledge and emphasis was to formulate a stable and functionally effective. The advantage of this gel is reduced adverse effects unlike synthetic drugs. The preliminary phytochemical screening of hydroalcoholic extract of Luffa acutangula seeds showed the presence of Carbohydrates, Amino acids, Proteins and tannins. The formulations were tested for the anti-acne activity by well diffusion method against Propionibacterium acnes. Results showed that the gels were non-irritant, stable and possess anti-acne activity. The efficacy when tested with a standard was almost same to that of marketed gel. This suggests that seeds of Luffa acutangula, have potential against acne causing bacteria and hence they can be used in topical anti-acne preparations and may address the antibiotic resistance of the bacteria.