The uses of flowers in Ayurveda treatment is known as Pushpayurveda or Floral therapy, which was discovered and practiced by Jain Mahaveer in 9th Century. The purpose of this study was aimed to collect and compile the classical references for uses of flowers in Ayurvedic treatment with special reference to Sushruta Samhita. According to the findings; the flowers have been recommended as both foods and medicines, the 30 flowers as vegetables while 100 flowers as medicinal purpose including the above 30 flowers, 5 flowers as poisonous. Other uses of flowers; to explain the causes, symptoms, severity and curability of diseases and also as a water purifier. Flowers can apply in both enteral and local application routes, in any types of dosage form as well as the garlands. The 201 classical preparations; 10 in Sutrasthana, 4 in Sharirasthana, 58 in Cikitsasthana, 30 in Kalpasthana and 100 in Uttarasthana. Flowers are responsible in both physical and mental health. Hence, it can be concluded that uses of flowers as medicine is not a new aspect of Ayurveda, but re-establishment of the concept of Pushpayurveda will be a new approach and value addition to the Ayurveda. Further, Acarya Sushruta emphasizes the properties of flowers are the same as the properties of their trees. Flowers can be collected without harming to the plant. Therefore, knowledge of Pushpayurveda will be helpful in Eco-friendly practice of Ayurveda.