Vishalyakarani or Tridax procumbens L is an Ayurvedic herb under asteraceae family with traditional use in wound healing purpose. It has many pharmacological properties including anti-microbial, anti-cancer, wound healing and anti-septic. The present study is a combined study consisting reviewing the anti-microbial spectrum of Tridax procumbens L. (Vishalyakarani) and quantification of flavonoids and phenols from its leaves especially from the Gandhamardhan hill, Odisha. This aims to equip researchers a useful data about the chemical contents of T. procumbens. The extract was prepared by using Soxhlet extractor and concentrated by distillation. Phytochemical analysis revealed the presence of flavonoids, phenols in extract and quantification by the high-throughput nine sixty well plate method. The review process yielded 748 research papers and after thorough discussion 81 papers were selected. The plant has many chemical compounds including flavonoids, essential oils, phenols and wide range of pharmacological activities from which we have included the anti-microbial activities. Historically and from Ramayana we have heard about the effectiveness of the Vishalyakarani in wound healing and in present review proves its worth. But there was a lack of quantification of two important phyto contents and it’s done by high-throughput 96-well plate method.