Helminth infections are among the most widespread infections in humans, distressing a huge population of the world. The fruit of Centratherum antheminticum (SYN-Vernonia anthelmintica, Family-Asteraceae) known in India as Kalijiri is one such herbal remedy for worm infestation, so to isolate anthelmintic compounds from Kalijiri by bioactivity guided fractionation. Caenorhabditis elegans was chosen as a model of organism to check anthelmintic activity because of its high degree of cell differentiation and specialization. Anthelmintic activity of methanol extract and its fraction was checked. Results of motility assay was analysed by statistical analysis. Result showed that methanolic extract and its fraction showed significant activity.